Fire opened from Syrian territory on military post at Mount Hermon.
The Israeli military said it fired into Syria on Monday morning in response to high trajectory force that struck the northern Golan Heights.
According to the Israel Defense Forceses, no one was wounded in the shooting from the Syrian side, which landed near a military post at Mount Hermon earlier Monday morning, and no damage was incurred.
Initially, the army said it responded with artillery fire. It later specified that it had fired a precision Tamuz anti-tank missile.
"The IDF reserves the right to act in any way and at any time it sees fit to defend the citizens of the State of Israel," the military said in a statement announcing the strike.
Mortar shells have exploded sporadically inside Israeli territory since the conflict in neighboring Syria began, sometimes causing minor damage. Israel believes most of the fire were errant shots but has at times accused Syria of aiming at Israeli targets. Israeli troops have returned fire on several occasions.
Israel captured the Golan Heights from Syria in the 1967 Six Day War. The frontier with Syria had been largely quiet for decades before the civil war erupted.
The exchange of fire on the Hermon came only several hours after the Israel Air Force jets struck two targets in the Gaza Strip.
The IDF Spokesperson's Unit said two Gazan terror targets suffered direct hits, one in the Gaza Strip's south, and the other in its center. The IDF reported direct hits, and said all aircraft had returned safely to base.
The strikes, the military said in a press release, were carried out in response to rocket fire toward Israel.
On Sunday evening, a rocket alert was sounded in Sha'ar Hanegev Regional council. No impact crater was found.
"The IDF will not tolerate attempts to harm Israeli citizens and IDF soldiers, and will act against anyone who uses terror against the State of Israel," the IDF said in the statement. "The terrorist organization Hamas is the address, and responsibility lies with it."
On April 21, six rockets fired from the Gaza Strip exploded in southern Israel. One of the missiles hit a street in the city of Sderot, causing damage but no injuries.
The Israel Air Force responded by attacking two targets in the southern Gaza Strip and other targets in the center.
On April 4, IDF jets struck Gaza targets shortly after a salvo of rockets was fired from Gaza into Israeli territory. The missiles landed in in open areas in the Sha'ar Hanegev Regional Council and caused no injuries.
Palestinians reported that two people were wounded by the Israeli air strike: a 30-year-old man and a one-year-old child.